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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    WB analysis using 80771-2-RR (same clone as 80771-2-PBS)

    Non-treated Jurkat cells, paclitaxel treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    IHC staining of human tonsillitis using 80771-2-RR (same clone as 80771-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    FC experiment of Jurkat using 80771-2-RR (same clone as 80771-2-PBS)

    1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR, Clone:230061A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80771-2-PBS in a different storage buffer formulation.

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Phospho-BCL2 (Ser70) Recombinant antibody, PBS Only

Phospho-BCL2 (Ser70) Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 80771-2-PBS
Clone No.230061A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

BCL-2, BCL2, 230061A8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80771-2-PBS targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)