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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    WB analysis using 80771-2-RR

    Non-treated Jurkat cells, paclitaxel treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    IHC staining of human tonsillitis using 80771-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    FC experiment of Jurkat using 80771-2-RR

    1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR, Clone:230061A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-BCL2 (Ser70) Recombinant antibody

Phospho-BCL2 (Ser70) Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 80771-2-RR
Clone No.230061A8
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

BCL-2, BCL2, 230061A8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inStaurosporine treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_3670487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)

Protocols

Product Specific Protocols
WB protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
IHC protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

NOD2 reduces the chemoresistance of melanoma by inhibiting the TYMS/PLK1 signaling axis

Authors - Fang Yun
View Article