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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    FC experiment of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.06 ug Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR, Clone:1L2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

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Phospho-CHEK2 (Thr68) Recombinant antibody, PBS Only

Phospho-CHEK2 (Thr68) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81740-1-PBS
Clone No.1L2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81740-1-PBS targets Phospho-CHEK2 (Thr68) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CHK2 checkpoint homolog (S. pombe)
Calculated molecular weight 61 kDa
Observed molecular weight65 kDa
GenBank accession numberBC004207
Gene Symbol CHEK2
Gene ID (NCBI) 11200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96017
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)