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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    WB analysis of PC-3 using 81740-1-RR

    Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • Western Blot (WB) analysis of HeLa cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    WB analysis of HeLa using 81740-1-RR

    Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR)

    FC experiment of PC-3 using 81740-1-RR

    1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.06 ug Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR, Clone:1L2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-CHEK2 (Thr68) Recombinant antibody

Phospho-CHEK2 (Thr68) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81740-1-RR
Clone No.1L2
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMMS treated PC-3 cells, UV treated HeLa cells
Positive FC (Intra) detected inMMS treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

81740-1-RR targets Phospho-CHEK2 (Thr68) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CHK2 checkpoint homolog (S. pombe)
Calculated molecular weight 61 kDa
Observed molecular weight65 kDa
GenBank accession numberBC004207
Gene Symbol CHEK2
Gene ID (NCBI) 11200
RRIDAB_2935575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96017
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Protocols

Product Specific Protocols
WB protocol for Phospho-CHEK2 (Thr68) antibody 81740-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

SPIN1 facilitates chemoresistance and HR repair by promoting Tip60 binding to H3K9me3

Authors - Yukun Wang
View Article