CoraLite® Plus 750-conjugated Phospho-CHEK2 (Thr68) Recombinant antibody
Phospho-CHEK2 (Thr68) Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
1L2
Cat no : CL750-81740
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MMS treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-81740 targets Phospho-CHEK2 (Thr68) in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | CHK2 checkpoint homolog (S. pombe) |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC004207 |
| Gene symbol | CHEK2 |
| Gene ID (NCBI) | 11200 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Phospho-CHEK2 (Thr68) antibody CL750-81740 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
