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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Polyclonal antibody (29715-1-AP)

    WB analysis using 29715-1-AP (same clone as 29715-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29715-1-AP (Phospho-Cofilin (Ser3) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as a loading control. This data was developed using the same antibody clone with 29715-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Cofilin (Ser3) Polyclonal antibody (29715-1-AP)

    IF Staining of HeLa using 29715-1-AP (same clone as 29715-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (29715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29715-1-PBS in a different storage buffer formulation.

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Phospho-Cofilin (Ser3) Polyclonal antibody, PBS Only

Phospho-Cofilin (Ser3) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29715-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29715-1-PBS targets Phospho-Cofilin (Ser3) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23528
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.