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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR)

    WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR)

    WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

    WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Jurkat cells using Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR)

    IHC staining of Jurkat using 80059-1-RR (same clone as 80059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Phospho-EIF4B (Ser406) Recombinant antibody, PBS Only

Phospho-EIF4B (Ser406) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80059-1-PBS
Clone No.5G14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80059-1-PBS targets Phospho-EIF4B (Ser406) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4B
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
RRIDAB_2918859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23588
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).