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  • Featured Product
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Phospho-EIF4B (Ser406) Recombinant antibody

Phospho-EIF4B (Ser406) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5G14

Cat no : 80059-1-RR

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR)

    WB analysis using 80059-1-RR

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Phospho-EIF4B (Ser406) Recombinant antibody (80059-1-RR)

    WB analysis of HeLa using 80059-1-RR

    WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells.

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Calyculin A treated HEK-293T cells, PC-3 cells, Calyculin A treated PC-3 cells, Calyculin A treated Hela cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80059-1-RR targets Phospho-EIF4B (Ser406) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4B
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC073154
Gene symbol EIF4B
Gene ID (NCBI) 1975
RRIDAB_2918859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).

Protocols

Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser406) antibody 80059-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Lactate-upregulated ARG2 expression induces cellular senescence in fibroblast-like synoviocytes of osteoarthritis via activating the mTOR/S6K1 signaling pathway

Authors - Yifan Huang
View Article