Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Calyculin A treated HEK-293T cells, PC-3 cells, Calyculin A treated PC-3 cells, Calyculin A treated Hela cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
80059-1-RR targets Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 4B |
| Calculated molecular weight | 611 aa, 69 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC073154 |
| Gene Symbol | EIF4B |
| Gene ID (NCBI) | 1975 |
| RRID | AB_2918859 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23588 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-EIF4B (Ser406) antibody 80059-1-RR | Download protocol |
| IHC protocol for Phospho-EIF4B (Ser406) antibody 80059-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Lactate-upregulated ARG2 expression induces cellular senescence in fibroblast-like synoviocytes of osteoarthritis via activating the mTOR/S6K1 signaling pathway |