CoraLite®594-conjugated Phospho-GSK3B (Ser9) Monoclonal antibody
Phospho-GSK3B (Ser9) Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1C9E2
Cat no : CL594-67558
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Calyculin A treated PC-3 cells |
| Positive FC (Intra) detected in | Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67558 targets Phospho-GSK3B (Ser9) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | glycogen synthase kinase 3 beta |
| Observed molecular weight | 48 kDa |
| GenBank accession number | NM_002093 |
| Gene symbol | GSK3B |
| Gene ID (NCBI) | 2932 |
| RRID | AB_2920159 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Phospho-GSK3B (Ser9) antibody CL594-67558 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
