Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
80340-1-PBS targets Phospho-Hamartin/TSC1 (Ser511) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.
| Tested Reactivity | mouse, human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tuberous sclerosis 1 |
| Calculated molecular weight | 130 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | NM_000368 |
| Gene Symbol | Hamartin/TSC1 |
| Gene ID (NCBI) | 7248 |
| RRID | AB_2918889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92574 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).