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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody, PBS Only

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3N6

Cat no : 80340-1-PBS

Synonyms

TSC1, TSC 1, TSC, LAM, KIAA0243

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80340-1-PBS targets Phospho-Hamartin/TSC1 (Ser511) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 1
Calculated molecular weight 130 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_000368
Gene symbol Hamartin/TSC1
Gene ID (NCBI) 7248
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).