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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3N6

Cat no : 80340-1-RR

Synonyms

TSC1, TSC 1, TSC, LAM, KIAA0243

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    WB analysis using 80340-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    WB analysis using 80340-1-RR

    Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-Hamartin/TSC1 (Ser511) Recombinant antibod (80340-1-RR)

    FC experiment of HeLa using 80340-1-RR

    1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

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Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80340-1-RR targets Phospho-Hamartin/TSC1 (Ser511) in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 1
Calculated molecular weight 130 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_000368
Gene symbol Hamartin/TSC1
Gene ID (NCBI) 7248
RRIDAB_2918889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).

Protocols

Product Specific Protocols
WB protocol for Phospho-Hamartin/TSC1 (Ser511) antibody 80340-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice

Authors - Xiaoxue Jiang
View Article