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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR)

    WB analysis of HeLa using 84739-2-RR (same clone as 84739-2-PBS)

    Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR)

    FC experiment of NIH/3T3 using 84739-2-RR (same clone as 84739-2-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.

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Phospho-Histone H3 (Thr3) Recombinant antibody, PBS Only

Phospho-Histone H3 (Thr3) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84739-2-PBS
Clone No.241897D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

HIST1H3A, 241897D9, Histone-H3, HistoneH3, Histone H3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84739-2-PBS targets Phospho-Histone H3 (Thr3) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight17 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.