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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR)

    WB analysis of HeLa using 84739-2-RR

    Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR)

    FC experiment of NIH/3T3 using 84739-2-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-Histone H3 (Thr3) Recombinant antibody

Phospho-Histone H3 (Thr3) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84739-2-RR
Clone No.241897D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Histone H3 (Thr3), p Histone H3, p Histone H3 (Thr3), p Histone H3 Thr3, p-Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84739-2-RR targets Phospho-Histone H3 (Thr3) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight17 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Thr3) antibody 84739-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols