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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody, PBS Only

Phospho-IkB Beta (Thr19/Ser23) Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241061B2

Cat no : 84403-1-PBS

Synonyms

IkB beta, IκB beta, IκB β, NFKBIB, IkB B

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Phospho-IkB Beta (Thr19/Ser23) Recombinant antibod (84403-1-RR)

    WB analysis of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    Non-treated HT-29 cells and TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84403-1-RR (Phospho-IkB Beta (Thr19/Ser23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84403-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using Phospho-IkB Beta (Thr19/Ser23) Recombinant antibod (84403-1-RR)

    FC experiment of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    1x10^6 TNF alpha and Calyculin A treated or untreated(black) HT-29 cells were intracellularly stained with 0.25 ug Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody (84403-1-RR, Clone:241061B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84403-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84403-1-PBS targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated molecular weight 356 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015528
Gene symbol IkB Beta
Gene ID (NCBI) 4793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.