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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody

Phospho-IkB Beta (Thr19/Ser23) Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241061B2

Cat no : 84403-1-RR

Synonyms

IkB beta, IκB beta, IκB β, NFKBIB, IkB B

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Phospho-IkB Beta (Thr19/Ser23) Recombinant antibod (84403-1-RR)

    WB analysis of HT-29 using 84403-1-RR

    Non-treated HT-29 cells and TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84403-1-RR (Phospho-IkB Beta (Thr19/Ser23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of HT-29 cells using Phospho-IkB Beta (Thr19/Ser23) Recombinant antibod (84403-1-RR)

    FC experiment of HT-29 using 84403-1-RR

    1x10^6 TNF alpha and Calyculin A treated or untreated(black) HT-29 cells were intracellularly stained with 0.25 ug Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody (84403-1-RR, Clone:241061B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive WB detected inTNF alpha and Calyculin A treated HT-29 cells
Positive FC (Intra) detected inTNF alpha and Calyculin A treated HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84403-1-RR targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated molecular weight 356 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015528
Gene symbol IkB Beta
Gene ID (NCBI) 4793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.

Protocols

Product Specific Protocols
WB protocol for Phospho-IkB Beta (Thr19/Ser23) antibody 84403-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols