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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR)

    WB analysis using 81667-2-RR (same clone as 81667-2-PBS)

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81667-2-RR (Phospho-JUN (Thr91/93) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81667-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR)

    FC experiment of NIH/3T3 using 81667-2-RR (same clone as 81667-2-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.13 ug Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR, Clone:241983H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81667-2-PBS in a different storage buffer formulation.

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Phospho-JUN (Thr91/93) Recombinant antibody, PBS Only

Phospho-JUN (Thr91/93) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81667-2-PBS
Clone No.241983H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

AP1, AP 1, JUN, c Jun, Activator protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81667-2-PBS targets Phospho-JUN (Thr91/93) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight48 kDa
GenBank accession numberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05412
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)