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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR)

    WB analysis using 81667-2-RR

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81667-2-RR (Phospho-JUN (Thr91/93) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR)

    FC experiment of NIH/3T3 using 81667-2-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.13 ug Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR, Clone:241983H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-JUN (Thr91/93) Recombinant antibody

Phospho-JUN (Thr91/93) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81667-2-RR
Clone No.241983H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

JUN (Thr91/93), p JUN (Thr91/93), Phospho JUN (Thr91/93), Phospho-JUN, Phospho-JUN Thr91/93

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inUV treated NIH/3T3 cells
Positive FC (Intra) detected inUV treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81667-2-RR targets Phospho-JUN (Thr91/93) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight48 kDa
GenBank accession numberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
RRIDAB_3670501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05412
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

Protocols

Product Specific Protocols
WB protocol for Phospho-JUN (Thr91/93) antibody 81667-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols