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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MEK2 (Thr394) Recombinant antibody, PBS Only

Phospho-MEK2 (Thr394) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241669E6

Cat no : 84556-1-PBS

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MEK2 (Thr394) Recombinant antibody (84556-1-RR)

    WB analysis using 84556-1-RR (same clone as 84556-1-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84556-1-RR (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84556-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84556-1-PBS targets Phospho-MEK2 (Thr394) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC018645
Gene symbol MEK2
Gene ID (NCBI) 5605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.