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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-MEK2 (Thr394) Recombinant antibody (84556-1-RR)

    WB analysis of NIH/3T3 using 84556-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84556-1-RR (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Phospho-MEK2 (Thr394) Recombinant antibody

Phospho-MEK2 (Thr394) Recombinant Antibody for WB, ELISA
Cat No. 84556-1-RR
Clone No.241669E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MEK2 (Thr394), Phospho MEK2 (Thr394), Phospho-MEK2, p-MEK2, p-MEK2 (Thr394)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84556-1-RR targets Phospho-MEK2 (Thr394) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 2
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC018645
Gene Symbol MEK2
Gene ID (NCBI) 5605
RRIDAB_3672065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Protocols

Product Specific Protocols
WB protocol for Phospho-MEK2 (Thr394) antibody 84556-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols