Phospho-P53 (Ser15) Monoclonal antibody
Phospho-P53 (Ser15) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1H6G1
Cat no : 67826-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated HT-29 cells, HT-29 cells, HEK-293 cells, HEK-293t cells, Etoposide treated HT-29 cells, Calyculin A treated HEK-293 cells, UV treated HEK-293T cells |
| Positive IF/ICC detected in | etoposide treated HT-29 cells |
| Positive FC (Intra) detected in | UV treated HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
67826-1-Ig targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tumor protein p53 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC003596 |
| Gene symbol | P53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_2918588 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-P53 (Ser15) antibody 67826-1-Ig | Download protocol |
| IF protocol for Phospho-P53 (Ser15) antibody 67826-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription | ||
Food Chem Toxicol Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system | ||
Front Pharmacol A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression. | ||
Toxicol Lett The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs] | ||
World Neurosurg N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway | ||
Front Pharmacol Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC |
