Phospho-P53 (Ser15) Monoclonal antibody, PBS Only (Capture)

Name: Phospho-P53 (Ser15) Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 67826-1-PBS

Phospho-P53 (Ser15) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H6G1

Review Product

Cat no : 67826-1-PBS

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

Filter:

AllWBIF/ICCFC (Intra)Cytometric bead array

WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)
Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
×
Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)
Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
×
Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)
Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
×
Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)
Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
×
Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
IF Staining of HT-29 using 67826-1-Ig (same clone as 67826-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (67826-1-Ig, Clone: 1H6G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
FC experiment of HT-29 using 67826-1-Ig (same clone as 67826-1-PBS)
1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
×
1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67826-1-PBS in a different storage buffer formulation.
Cytometric bead array in cell lysate using MP50182-1
Cytometric bead array in cell lysate using MP50182-1, Phospho-P53 (Ser15) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67826-1-PBS. Detection antibody: 60283-2-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.
Cytometric bead array in cell lysate using MP50183-1
Cytometric bead array in cell lysate using MP50183-1, Phospho-P53 (Ser15&46) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67826-1-PBS. Detection antibody: 67900-1-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

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Cat No. 67826-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67826-1-PBS targets Phospho-P53 (Ser15) as part of a matched antibody pair:

MP50182-1: 67826-1-PBS capture and 60283-2-PBS detection (validated in Cytometric bead array)

MP50183-1: 67826-1-PBS capture and 67900-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	tumor protein p53
Calculated molecular weight	44 kDa
Observed molecular weight	53 kDa
GenBank accession number	BC003596
Gene symbol	P53
Gene ID (NCBI)	7157
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

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Species Reactivity

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Conjugate

Conjugate

Phospho-P53 (Ser15) Monoclonal antibody, PBS Only (Capture)

Phospho-P53 (Ser15) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Validation Data Gallery

WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)

WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)

WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)

WB analysis using 67826-1-Ig (same clone as 67826-1-PBS)

IF Staining of HT-29 using 67826-1-Ig (same clone as 67826-1-PBS)

FC experiment of HT-29 using 67826-1-Ig (same clone as 67826-1-PBS)

Cytometric bead array in cell lysate using MP50182-1

Cytometric bead array in cell lysate using MP50183-1

Tested Applications

Recommended dilution

Product Information

Background Information