Phospho-P53 (Ser15) Recombinant antibody, PBS Only

Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells and non-treated HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.