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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-P53 (Ser15) Recombinant antibody

Phospho-P53 (Ser15) Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2J21

Cat no : 80195-1-RR

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser15) Recombinant antibody (80195-1-RR)

    WB analysis using 80195-1-RR

    Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser15) Recombinant antibody (80195-1-RR)

    WB analysis using 80195-1-RR

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser15) Recombinant antibody (80195-1-RR)

    IF Staining of HT-29 using 80195-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells and non-treated HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using Phospho-P53 (Ser15) Recombinant antibody (80195-1-RR)

    FC experiment of A431 using 80195-1-RR

    1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive WB detected inetoposide treated HT-29 cells, UV treated A431 cells
Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80195-1-RR targets Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol P53
Gene ID (NCBI) 7157
RRIDAB_2918873
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

Protocols

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 80195-1-RRDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 80195-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Involvement of JNK signaling in Aspergillus fumigatus-induced inflammatory factors release in bronchial epithelial cells

Authors - Xiao Cui
View Article

Cells

Concurrent Activation of Both Survival-Promoting and Death-Inducing Signaling by Chloroquine in Glioblastoma Stem Cells: Implications for Potential Risks and Benefits of Using Chloroquine as Radiosensitizer

Authors - Andreas Müller
View Article