CoraLite® Plus 647-conjugated Phospho-P53 (Ser15) Recombinant antibody
Phospho-P53 (Ser15) Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2J21
Cat no : CL647-80195
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | etoposide treated HT-29 cells |
Positive FC (Intra) detected in | UV treated A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-80195 targets Phospho-P53 (Ser15) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | tumor protein p53 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC003596 |
Gene symbol | P53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Phospho-P53 (Ser15) antibody CL647-80195 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |