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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated Phospho-P53 (Ser15) Recombinant antibody

Phospho-P53 (Ser15) Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2J21

Cat no : CL555-80195

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite®555-conjugated Phospho-P53 (Ser15) Recomb (CL555-80195)

    IF Staining of HT-29 using CL555-80195

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using CoraLite®555 Phospho-P53 (Ser15) antibody (CL555-80195, Clone: 2J21 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®555-conjugated Phospho-P53 (Ser15) Recomb (CL555-80195)

    FC experiment of A431 using CL555-80195

    1X10^6 A431 cells untreated (dashed line) or UV treated A431 cells (red) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-P53 (Ser15) (CL555-80195, Clone:2J21), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated A431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80195 targets Phospho-P53 (Ser15) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

Protocols

Product Specific Protocols
IF protocol for CL555 Phospho-P53 (Ser15) antibody CL555-80195Download protocol
Standard Protocols
Click here to view our Standard Protocols