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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-P53 (Ser46) Monoclonal antibody, PBS Only (Detector)

Phospho-P53 (Ser46) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D10A12

Cat no : 67900-1-PBS

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    WB analysis using 67900-1-Ig (same clone as 67900-1-PBS)

    Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 67900-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    WB analysis using 67900-1-Ig (same clone as 67900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 67900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    IHC staining of human colon cancer using 67900-1-Ig (same clone as 67900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    IF Staining of HT-29 using 67900-1-Ig (same clone as 67900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser46) antibody (67900-1-Ig, Clone: 1D10A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67900-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    FC experiment of HT-29 using 67900-1-Ig (same clone as 67900-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Phospho-P53 (Ser46) (67900-1-Ig, Clone:1D10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67900-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50183-1

    Cytometric bead array in cell lysate using MP50183-1

    Cytometric bead array in cell lysate using MP50183-1, Phospho-P53 (Ser15&46) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67826-1-PBS. Detection antibody: 67900-1-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

  • Cytometric bead array in cell lysate using MP50184-1

    Cytometric bead array in cell lysate using MP50184-1

    Cytometric bead array in cell lysate using MP50184-1, Phospho-P53 (Ser46) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60283-2-PBS. Detection antibody: 67900-1-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67900-1-PBS targets Phospho-P53 (Ser46) as part of a matched antibody pair:

MP50183-1: 67826-1-PBS capture and 67900-1-PBS detection (validated in Cytometric bead array)

MP50184-1: 60283-2-PBS capture and 67900-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol P53
Gene ID (NCBI) 7157
RRIDAB_2918656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)