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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-P53 (Ser46) Monoclonal antibody

Phospho-P53 (Ser46) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D10A12

Cat no : 67900-1-Ig

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    WB analysis using 67900-1-Ig

    Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    WB analysis using 67900-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    IHC staining of human colon cancer using 67900-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67900-1-Ig (Phospho-P53 (Ser46) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    IF Staining of HT-29 using 67900-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser46) antibody (67900-1-Ig, Clone: 1D10A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HT-29 cells using Phospho-P53 (Ser46) Monoclonal antibody (67900-1-Ig)

    FC experiment of HT-29 using 67900-1-Ig

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Phospho-P53 (Ser46) (67900-1-Ig, Clone:1D10A12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHT-29 cells, Calyculin A treated HT-29 cells, etoposide treated HT-29 cells, UV treated A431 cells, Calyculin A treated HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67900-1-Ig targets Phospho-P53 (Ser46) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol P53
Gene ID (NCBI) 7157
RRIDAB_2918656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

Protocols

Product Specific Protocols
WB protocol for Phospho-P53 (Ser46) antibody 67900-1-IgDownload protocol
IHC protocol for Phospho-P53 (Ser46) antibody 67900-1-IgDownload protocol
IF protocol for Phospho-P53 (Ser46) antibody 67900-1-IgDownload protocol
FC protocol for Phospho-P53 (Ser46) antibody 67900-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer Res Clin Oncol

Urocortin-1 promotes colorectal cancer cell migration and proliferation and inhibits apoptosis via inhibition of the p53 signaling pathway

Authors - Xiaolan Guo
View Article