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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) Monoclonal antibody

Phospho-P53 (Ser46) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D10A12

Cat no : CL488-67900

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) (CL488-67900)

    IF Staining of HT-29 using CL488-67900

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using CoraLite® Plus 488 Phospho-P53 (Ser46) antibody (CL488-67900, Clone: 1D10A12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) (CL488-67900)

    FC experiment of HT-29 using CL488-67900

    1X10^6 HT-29 cells untreated (dashed lines) or treated with UV (red) were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-P53 (Ser46) (CL488-67900, Clone:1D10A12), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated HT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67900 targets Phospho-P53 (Ser46) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-P53 (Ser46) antibody CL488-67900Download protocol
Standard Protocols
Click here to view our Standard Protocols