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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant an (85044-1-RR)

    WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant an (85044-1-RR)

    WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

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Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant antibody, PBS Only

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85044-1-PBS
Clone No.242123D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

PAK1, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85044-1-PBS targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight 553 aa, 62 kDa
Observed molecular weight61 kDa
GenBank accession numberBC109299
Gene Symbol PAK1
Gene ID (NCBI) 5058
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13153
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)