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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant an (85044-1-RR)

    WB analysis using 85044-1-RR

    Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant an (85044-1-RR)

    WB analysis using 85044-1-RR

    Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant antibody

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85044-1-RR
Clone No.242123D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAK1 (Ser144), PAK1 (Ser144)/PAK2 (Ser141), PAK2 (Ser141), Phospho-PAK1, Phospho-PAK1 (Ser144)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpervanadate treated C6 cells, LPS treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85044-1-RR targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight 553 aa, 62 kDa
Observed molecular weight61 kDa
GenBank accession numberBC109299
Gene Symbol PAK1
Gene ID (NCBI) 5058
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)

Protocols

Product Specific Protocols
WB protocol for Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody 85044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols