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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H7A11

Cat no : 60597-1-Ig

Synonyms

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig

    Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, PC-3 cells, Jurkat cells, Calyculin A treated Jurkat cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60597-1-Ig targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight140 kDa
GenBank accession numberBC126354
Gene symbol PERK
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Protocols

Product Specific Protocols
WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 60597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols