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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

    Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody (60597-1-Ig)

    WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

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Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody, PBS Only

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60597-1-PBS
Clone No.3H7A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60597-1-PBS targets Phospho-PERK/EIF2AK3 (Ser719) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight140 kDa
GenBank accession numberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)