Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
81575-2-PBS targets Phospho-PKC Alpha (Thr638) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase C, alpha |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | AK055431 |
| Gene Symbol | PKC Alpha |
| Gene ID (NCBI) | 5578 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P17252 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)