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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PKC Alpha (Thr638) Recombinant antibody (81575-2-RR)

    WB analysis using 81575-2-RR

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Phospho-PKC Alpha (Thr638) Recombinant antibody

Phospho-PKC Alpha (Thr638) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81575-2-RR
Clone No.241844A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-PKC α Thr638, Phospho-PKC beta (Thr641), Phospho-PKC, p-PKC, p-PKC Alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, λ phosphatase treated THP-1 cells

The antibody recognizes PKC Beta-II protein phosphorylated at Thr641.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81575-2-RR targets Phospho-PKC Alpha (Thr638) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase C, alpha
Calculated molecular weight 77 kDa
Observed molecular weight77 kDa
GenBank accession numberAK055431
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

Protocols

Product Specific Protocols
WB protocol for Phospho-PKC Alpha (Thr638) antibody 81575-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols