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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PLA2G4A (Ser505) Polyclonal antibody (28925-1-AP)

    WB analysis using 28925-1-AP (same clone as 28925-1-PBS)

    Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight. This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-PLA2G4A (Ser505) Polyclonal antibody (28925-1-AP)

    IF Staining of HeLa using 28925-1-AP (same clone as 28925-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

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Phospho-PLA2G4A (Ser505) Polyclonal antibody, PBS Only

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28925-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28925-1-PBS targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2918213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)