Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
81180-2-PBS targets Phospho-Paxillin (Tyr118) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | paxillin |
| Calculated molecular weight | 591 aa, 65 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC136787 |
| Gene Symbol | Paxillin |
| Gene ID (NCBI) | 5829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49023 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)