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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Paxillin (Tyr118) Recombinant antibody (81180-2-RR)

    WB analysis using 81180-2-RR

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 81180-2-RR (Phospho-Paxillin (Tyr118) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Phospho-Paxillin (Tyr118) Recombinant antibody

Phospho-Paxillin (Tyr118) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81180-2-RR
Clone No.241063E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inpervanadate treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81180-2-RR targets Phospho-Paxillin (Tyr118) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name paxillin
Calculated molecular weight 591 aa, 65 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC136787
Gene Symbol Paxillin
Gene ID (NCBI) 5829
RRIDAB_3670493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)

Protocols

Product Specific Protocols
WB protocol for Phospho-Paxillin (Tyr118) antibody 81180-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols