Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
82309-1-PBS targets Phospho-RASGRF1 (Ser927) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC040275 |
| Gene Symbol | RASGRF1 |
| Gene ID (NCBI) | 5923 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13972 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)