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Phospho-RASGRF1 (Ser927) Recombinant antibody

Phospho-RASGRF1 (Ser927) Recombinant Antibody for WB, ELISA
Cat No. 82309-1-RR
Clone No.5K3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

p RASGRF1, p RASGRF1 (Ser927), p RASGRF1 Ser927, Phospho RASGRF1, Phospho RASGRF1 (Ser927)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inForskolin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Calculated molecular weight 134 kDa
Observed molecular weight145 kDa
GenBank accession numberBC040275
Gene Symbol RASGRF1
Gene ID (NCBI) 5923
RRIDAB_3085358
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13972
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)

Protocols

Product Specific Protocols
WB protocol for Phospho-RASGRF1 (Ser927) antibody 82309-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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