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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RASGRF1 (Ser927) Recombinant antibody

Phospho-RASGRF1 (Ser927) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5K3

Cat no : 82309-1-RR

Synonyms

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, Phospho-RASGRF1 (Ser927), PP13187, Ras GRF1, RASGRF1, Ras-GRF1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-RASGRF1 (Ser927) Recombinant antibody (82309-1-RR)

    WB analysis of HeLa using 82309-1-RR

    Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control.

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Tested Applications

Positive WB detected inForskolin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Calculated molecular weight 134 kDa
Observed molecular weight145 kDa
GenBank accession numberBC040275
Gene symbol RASGRF1
Gene ID (NCBI) 5923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)

Protocols

Product Specific Protocols
WB protocol for Phospho-RASGRF1 (Ser927) antibody 82309-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols