Validation Data Gallery
Tested Applications
| Positive WB detected in | Forskolin treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC040275 |
| Gene Symbol | RASGRF1 |
| Gene ID (NCBI) | 5923 |
| RRID | AB_3085358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RASGRF1 (Ser927) antibody 82309-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |