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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser161) Monoclonal antibody

Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2G1

Cat no : CL488-66854

Synonyms

RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser16 (CL488-66854)

    IF Staining of U2OS using CL488-66854

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser16 (CL488-66854)

    IF Staining of HeLa using CL488-66854

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser16 (CL488-66854)

    FC experiment of HEK-293T using CL488-66854

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive IF/ICC detected inCalyculin A treated HeLa cells, U2OS cells
Positive FC (Intra) detected inCalyculin A treated HEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66854 targets Phospho-RIPK1 (Ser161) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
Gene ID (NCBI) 8737
RRIDAB_2919380
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-RIPK1 (Ser161) antibody CL488-66854Download protocol
Standard Protocols
Click here to view our Standard Protocols