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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

    Non-treated and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

    Non-treated and PDGF (HZ-1215) treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236)) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    IF Staining of HeLa using 80130-2-RR (same clone as 80130-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) antibody (80130-2-RR, Clone: 241018D12) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004). This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    FC experiment of HeLa using 80130-2-RR (same clone as 80130-2-PBS)

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody (80130-2-RR, Clone: 241018D12, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80130-2-PBS
Clone No.241018D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80130-2-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Observed molecular weight35 kDa
GenBank accession numberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)