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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis using 80130-2-RR

    Non-treated and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis of NIH/3T3 using 80130-2-RR

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    WB analysis using 80130-2-RR

    Non-treated and PDGF (HZ-1215) treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236)) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    IF Staining of HeLa using 80130-2-RR

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) antibody (80130-2-RR, Clone: 241018D12) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) Recombin (80130-2-RR)

    FC experiment of HeLa using 80130-2-RR

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody (80130-2-RR, Clone: 241018D12, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA .

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Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80130-2-RR
Clone No.241018D12
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells
Positive IF/ICC detected in100 nM Calyculin A (30 minutes) treated HeLa cells
Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Observed molecular weight35 kDa
GenBank accession numberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_3670467
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)

Protocols

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RRDownload protocol
IF protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling

Authors - Kunxiang Gong
View Article