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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis of NIH/3T3 using 80575-3-RR (same clone as 80575-3-PBS)

    NIH/3T3 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis of PC-12 using 80575-3-RR (same clone as 80575-3-PBS)

    PC-12 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (80575-3-RR)

    WB analysis using 80575-3-RR (same clone as 80575-3-PBS)

    HEK-293 cells treated with 10% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80575-3-PBS in a different storage buffer formulation.

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Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80575-3-PBS
Clone No.242048C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80575-3-PBS targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)