Validation Data Gallery
Tested Applications
Positive WB detected in | 20% fetal bovine serum after serum free NIH/3T3 cells, 10% fetal bovine serum after serum free HEK-293 cells, 20% fetal bovine serum after serum free PC-12 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80575-3-RR targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | ribosomal protein S6 |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC000524 |
Gene Symbol | RPS6 |
Gene ID (NCBI) | 6194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P62753 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 80575-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |