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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT5A (Ser726) Recombinant antibody

Phospho-STAT5A (Ser726) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240709D6

Cat no : 80138-2-RR

Synonyms

p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT5A (Ser726) Recombinant antibody (80138-2-RR)

    WB analysis using 80138-2-RR

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Tested Applications

Positive WB detected inGM-CSF treated TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80138-2-RR targets Phospho-STAT5A (Ser726) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight90-95 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT5A (Ser726) antibody 80138-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols