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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinan (80138-2-RR)

    WB analysis using 80138-2-RR (same clone as 80138-2-PBS)

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80138-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinan (80138-2-RR)

    IF Staining of TF-1 using 80138-2-RR (same clone as 80138-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80138-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinan (80138-2-RR)

    IF Staining of TF-1 using 80138-2-RR (same clone as 80138-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80138-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinan (80138-2-RR)

    FC experiment of TF-1 using 80138-2-RR (same clone as 80138-2-PBS)

    1x10^6 untreated TF-1 cells (dash lines) and 100 nM Calyculin A (30 minutes) treated TF-1 cells (full lines) were intracellularly stained with 0.4 μg Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant antibody (80138-2-RR, Clone: 240709D6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 80138-2-PBS in a different storage buffer formulation.

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Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant antibody, PBS Only

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80138-2-PBS
Clone No.240709D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

STAT5A, STAT5, MGF, 240709D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80138-2-PBS targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight90-95 kDa
GenBank accession numberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42229
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)