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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Phospho-STK11/LKB1 (Thr189) Recombinant antibody

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5J9

Cat no : CL647-80127

Synonyms

LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 647-conjugated Phospho-STK11/LKB1 ( (CL647-80127)

    IF Staining of PC-3 using CL647-80127

    Immunofluorescent analysis of (-20°C Ethanol) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using CoraLite® Plus 647 Phospho-STK11/LKB1 (Thr189) antibody (CL647-80127, Clone: 5J9) at dilution of 1:100.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 647-conjugated Phospho-STK11/LKB1 ( (CL647-80127)

    FC experiment of PC-3 using CL647-80127

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STK11/LKB1 (Thr189) (CL647-80127, Clone:5J9) , or 0.06 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inCalyculin A treated PC-3 cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80127 targets Phospho-STK11/LKB1 (Thr189) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC007981
Gene symbol LKB1
Gene ID (NCBI) 6794
RRIDAB_2920331
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Phospho-STK11/LKB1 (Thr189) antibody CL647-80127Download protocol
Standard Protocols
Click here to view our Standard Protocols