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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Phospho-SYK (Tyr323) Recombinant antibody

Phospho-SYK (Tyr323) Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H24

Cat no : CL488-83331

Synonyms

SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CoraLite® Plus 488-conjugated Phospho-SYK (Tyr323) (CL488-83331)

    IF Staining of Ramos using CL488-83331

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using CoraLite® Plus 488 Phospho-SYK (Tyr323) antibody (CL488-83331, Clone: 2H24 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inpervanadate treated Ramos cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83331 targets Phospho-SYK (Tyr323) in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spleen tyrosine kinase
Calculated molecular weight 72 kDa
Observed molecular weight70 kDa
GenBank accession numberBC001645
Gene symbol SYK
Gene ID (NCBI) 6850
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-SYK (Tyr323) antibody CL488-83331Download protocol
Standard Protocols
Click here to view our Standard Protocols