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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR)

    WB analysis using 80101-7-RR (same clone as 80101-7-PBS)

    Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80101-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR)

    FC experiment of SH-SY5Y using 80101-7-RR (same clone as 80101-7-PBS)

    1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80101-7-PBS in a different storage buffer formulation.

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Phospho-TAU (Thr231) Recombinant antibody, PBS Only

Phospho-TAU (Thr231) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80101-7-PBS
Clone No.240632B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

MAPT, TAU, Microtubule-associated protein tau, MAPTL, DDPAC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80101-7-PBS targets Phospho-TAU (Thr231) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight50-80 kDa
GenBank accession numberBC000558
Gene Symbol TAU
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10636
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.