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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TAU (Thr231) Recombinant antibody

Phospho-TAU (Thr231) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240632B1

Cat no : 80101-7-RR

Synonyms

p TAU, p TAU (Thr231), Phospho TAU, Phospho TAU (Thr231), Phospho-TAU

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR)

    WB analysis using 80101-7-RR

    Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Tested Applications

Positive WB detected inSH-SY5Y cells, λ phosphatase treated SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80101-7-RR targets Phospho-TAU (Thr231) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight50-80 kDa
GenBank accession numberBC000558
Gene symbol TAU
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Protocols

Product Specific Protocols
WB protocol for Phospho-TAU (Thr231) antibody 80101-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols